- Description of Target:
- EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
- Gene Symbol:
- Official Gene Full Name:
- Early endosome antigen 1
- NCBI Gene Id:
- Alias Symbols:
- MST105; ZFYVE2; MSTP105
- Tissue Tool:
- Find tissues and cell lines supported to express EEA1.
- Protein Accession# :
- Nucleotide Accession#:
- Swissprot Id:
- Protein Name:
- Early endosome antigen 1
- Protein Size (# AA):
- Molecular Weight:
- WB, IP
- The immunogen for anti-EEA1 antibody: synthetic peptide directed towards the N terminal of human EEA1
- Product Format:
- Lyophilized powder
- Affinity Purified
- Complete computational species homology data:
- EEA1 antibody - N-terminal region (ARP30074_P050)
- Predicted Homology Based on Immunogen Sequence:
- Human: 100%; Guinea pig: 92%; Mouse: 92%; Bovine: 91%; Horse: 91%; Rat: 91%; Rabbit: 84%; Pig: 83%; Dog: 75%
- Species Reactivity:
- Bovine, Dog, Guinea pig, Horse, Human, Mouse, Pig, Rabbit, Rat
- Datasheets / Downloads:
- Printable datasheet for
anti-EEA1 antibody- ARP30074_P050
- Peptide Sequence:
- Synthetic peptide located within the following region: LTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSAS
- Blocking Peptide:
- For anti-EEA1 antibody is Catalog # AAP30074 (Previous Catalog # AAPH00250)
- Key Reference:
- Fratti,R.A., et al., (2003) J. Biol. Chem. 278 (47), 46961-46967
- Reconstitution and Storage:
- Add 50 ul of distilled water. Final anti-EEA1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20C. Avoid repeat freeze-thaw cycles.
Aviva Systems Biology is the original manufacturer of this EEA1 antibody (ARP30074_P050)
Click here to view the EEA1 antibody Western Blot Protocol
Product Datasheet Link: EEA1 antibody (ARP30074_P050)
WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml
ELISA Titer: 1:312500
Positive Control: Placenta
Western Blot image:
Description of Target: EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
Questions pertaining to this data can be directed to email@example.com
Aviva Systems Biology’s EEA1 antibody (ARP30074_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at firstname.lastname@example.org.
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Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Species+tissue/cell type: Rat brain homogenate
How many ug’s of tissue/cell lysate run on the gel: 500 ug rat brain homogenate
IP antibody: 6 ug
Primary antibody dilution: 1:500
Secondary antibody: Goat anti-rabbit Alexa-Fluor 594
Secondary antibody dilution: 1:5000
IP Protocol (Protein G coupled antibody):
Brain tissues were homogenized in a buffer containing 125 mM NaCl, 20mM Tris, pH7.4,
1mM MagCl2, 2% CHAP, 10% Glycerol, and protease inhibitor mirture.
Incubate on ice for 30 min.
Spin down 10 min at 14k rpm.
Add Protein G preclearance for 30 min on ice with rocking.
Spin down 5 min.
Add 6-12 of pre-coupled antibody-Protein G and rock 2 h on ice.
Spin down and wash 6 times.
Boil sample in sample buffer with beta-ME for 10 min.
Resolve on 4-20% Pierce Precise Protein gels.
Blot overnight with 2ug/ml antibodies.
Wash 2x7 min in TBS-tween.
Add secondary antibody (LiCor) for 1.5h.
Take images using LiCor.