NFKBIB antibody - middle region (ARP34381_P050)

Catalog#: ARP34381_P050

• Size: 50ug

Human 721_B

• Description of Target: Inhibition of NFkappa-B activity by the hepatitis C virus core protein might be related to its physical interaction with and interrupted nuclear localization of IKKbeta. Increased nuclear factor-kappaB (NF-kB) activity in the amnion during labor is associated with an increase in the expression of NF-kBp65 and of the NF-kB binding proteins IkBa, IkBb-1 and IkBb-2. IkappaBbeta may be a novel target for transcription factors of the HMG-box SRY/Sox family and imply a potential role for NF-kappaB/IkappaBbeta in spermatogenesis. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-766 BM449613.1 1-766 767-1189 BU634404.1 18-440 c 1190-1198 AI684894.1 1-9 c
• Gene Symbol: NFKBIB
• Official Gene Full Name: Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• Alias Symbols: IKBB; TRIP9
• Protein Accession#: NP_002494
• Nucleotide Accession#: NM_002503
• Swissprot Id: Q15653
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 356
• Molecular Weight: 38kDa
• Application: WB
• Partner Proteins: IKBKB, MCC, RELA, RELA, BTRC, CD3EAP, CHUK, CUL1, IKBKB, IKBKG, KPNA2, LRPPRC, MCC, MTIF2, NFKB1, NFKB2, NFKBIA, NGFR, NKIRAS1, NKIRAS2, PDCD2, POLR1A, POLR1B, POLR1C, POLR1D, POLR1E, POLR2H, POLR2L, RASAL2, REL, RELA, RXRA, SKP1, BTRC, CHUK, IKBKB, NKIRAS1, REL, RELA, RXRA, Thrb
• Immunogen: The immunogen for anti-NFKBIB antibody: synthetic peptide directed towards the middle region of human NFKBIB
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Horse: 100%; Mouse: 100%; Rat: 100%; Bovine: 92%
• Peptide Sequence: Synthetic peptide located within the following region: PILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRS
• Blocking Peptide: For anti-NFKBIB antibody is Catalog # AAP34381 (Previous Catalog # AAPY00223)
• Key Reference: Kerppola,T.K. (er) Ann. Rheum. Dis. (2008) In press
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-NFKBIB antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: NFKBIB antibody tested with Human 721_B_Lymphoblast (ARP34381_P050)

Aviva Systems Biology is the original manufacturer of this NFKBIB antibody (ARP34381_P050)

Click here to view the NFKBIB antibody Western Blot Protocol

Product Datasheet Link: NFKBIB antibody (ARP34381_P050)

WB Suggested Anti-NFKBIB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B

Western Blot image:


Description of Target: Inhibition of NFkappa-B activity by the hepatitis C virus core protein might be related to its physical interaction with and interrupted nuclear localization of IKKbeta. Increased nuclear factor-kappaB (NF-kB) activity in the amnion during labor is associated with an increase in the expression of NF-kBp65 and of the NF-kB binding proteins IkBa, IkBb-1 and IkBb-2. IkappaBbeta may be a novel target for transcription factors of the HMG-box SRY/Sox family and imply a potential role for NF-kappaB/IkappaBbeta in spermatogenesis. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-766 BM449613.1 1-766 767-1189 BU634404.1 18-440 c 1190-1198 AI684894.1 1-9 c

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Aviva Systems Biology’s NFKBIB antibody (ARP34381_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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