IL6ST Antibody - middle region (ARP63223_P050)

Catalog No: ARP63223_P050

WB Suggested Anti-IL6ST Antibody
Titration: 1.0 ug/ml
Positive Control: MDA-MB-435S Whole Cell

Host: Rabbit
Target: IL6ST
Positive control (+): Mouse Kidney (M-KI)
Negative control (-): Mouse Small Intestine (M-IN)
Antibody concentration: 1ug/ml

Rabbit Anti-IL6ST Antibody
Catalog Number: ARP63223_P050
Formalin Fixed Paraffin Embedded Tissue: Human Thyroid Tissue
Observed Staining: Plasma membrane and cytoplasm in follicular cells
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Rabbit Anti-IL6ST Antibody
Catalog Number: ARP63223_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Plasma membrane
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Rabbit: 77%; Rat: 86%
• Peptide Sequence: Synthetic peptide located within the following region: FTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKV
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-IL6ST (ARP63223_P050) antibody is Catalog # AAP63223
• Subunit: beta

Target Info

• Gene Symbol: IL6ST
• Gene Full Name: Interleukin 6 signal transducer (gp130, oncostatin M receptor)
• Alias Symbols: CD130, GP130, HIES4, CDW130, IL-6RB, sGP130
• NCBI Gene Id: 3572
• Protein Name: Interleukin-6 receptor subunit beta
• Description of Target: The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis.
• Uniprot ID: P40189-2
• Protein Accession #: NP_786943
• Nucleotide Accession #: NM_175767
• Protein Size (# AA): 329
• Molecular Weight: 35kDa
• Protein Interactions: SGTB; KCNIP3; UBQLN1; UBC; MAGEA11; SOCS3; PTPN11; IL31RA; CDK9; LIF; OSMR; PIK3CG; IL6ST; CTF1; TLE1; AR; ERBB3; PRKCD; TYK2; PTPN6; PLAUR; OSM; VAV1; ERBB2; LIFR; IL6R; JAK1; IL6; HCK; CNTFR; CNTF; STAT3; SHC1; PIK3R1; JAK2; GRB2;