DLAT Antibody - C-terminal region (ARP41995_P050)

Catalog No: ARP41995_P050

WB Suggested Anti-DLAT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysateDLAT is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Pig, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DLAT
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Human: 100%; Mouse: 100%; Pig: 92%; Rat: 100%; Zebrafish: 92%
• Peptide Sequence: Synthetic peptide located within the following region: DVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILA
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-DLAT (ARP41995_P050) antibody is Catalog # AAP41995 (Previous Catalog # AAPS11312)
• Sample Type Confirmation:

  DLAT is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Reference: Korotchkina,L.G. (2008) FEBS Lett. 582 (3), 468-472

Target Info

• Gene Symbol: DLAT
• Gene Full Name: Dihydrolipoamide S-acetyltransferase
• Alias Symbols: E2, PBC, DLTA, PDCE2, PDC-E2
• NCBI Gene Id: 1737
• Protein Name: Dihydrolipoamide S-acetyltransferase EMBL AAH39084.1
• Description of Target: DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2696 AK057299.1 1-2696 2697-3933 BC039084.1 2066-3302 3934-4322 DB551427.1 98-486
• Uniprot ID: Q86YI5
• Protein Accession #: NP_001922
• Nucleotide Accession #: NM_001931
• Protein Size (# AA): 647
• Molecular Weight: 69kDa
• Protein Interactions: MDM2; UBC; ADRB2; PRKD3; PDHX; MMGT1; MRPL1; ACAD9; SYNCRIP; PDHA2; PDHA1; OGDH; NDUFS4; HSPA1L; DLD; COX5B; COPS5; CUL1; SIRT7; TSG101; KIAA0101; PSMA3; PDK4; PDK3; PDK2; PDK1; PDHB;