TIAL1 Antibody - C-terminal region (AVARP02048_T100)

Catalog No: AVARP02048_T100

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Protein may be acetylated or phosphorylated.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TIAL1
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 85%; Rabbit: 100%; Rat: 85%; Zebrafish: 76%
• Peptide Sequence: Synthetic peptide located within the following region: WNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIPPPNQAGYGMASYQTQ
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-TIAL1 (AVARP02048_T100) antibody is Catalog # AAP30620 (Previous Catalog # AAPP01273)
• Sample Type Confirmation:

  TIAL1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Enhanced Validation:

  Relative Expression (Western Blot)

• Reference: Esclatine,A., et al., (2004) J. Virol. 78 (16), 8582-8592

Target Info

• Gene Symbol: TIAL1
• Gene Full Name: TIA1 cytotoxic granule-associated RNA binding protein-like 1
• Alias Symbols: TCBP, TIAR
• NCBI Gene Id: 7073
• Protein Name: Nucleolysin TIAR
• Description of Target: The protein encoded by TIAL1 is a member of a family of RNA-binding proteins and possesses nucleolytic activity against cytotoxic lymphocyte target cells. The gene product is a cytotoxic granule-associated protein and has been shown to bind specifically to poly(A) homopolymers and to fragment DNA in permeabilized target cells. It has been suggested that members of this protein family may be involved in the induction of apoptosis. One isoform contains a lysosome-targeting motif in its C-terminal auxiliary domain; however, alternative splicing results in a T-cluster DNA-binding isoform, differing at the C-terminus where a hydrophobic sequence replaces the lysosome-targeting motif.
• Uniprot ID: Q01085
• Protein Accession #: NP_003243
• Nucleotide Accession #: NM_003252
• Protein Size (# AA): 375
• Molecular Weight: 42 kDa
• Protein Interactions: SUMO2; UBC; RPA3; RPA2; RPA1; BMI1; SOX2; MAPK1; GADD45A; VCAM1; ITGA4; FN1; MRPL53; SARNP; UFM1; SBDS; MRPS28; TOMM40; ACAA2; GNB2L1; SF3B4; SCAMP3; SCO2; ALDH5A1; VDAC1; UBE2D2; THY1; SOD1; MBNL1; CAND1; DCUN1D1; COPS5; COPS6; CUL1; CUL2; CUL3; CUL4A; C