IMPDH2 Antibody - N-terminal region (ARP54365_P050)

Catalog No: ARP54365_P050

Host: Rabbit
Target Name: IMPDH2
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 3ug/ml

WB Suggested Anti-IMPDH2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysateIMPDH2 is supported by BioGPS gene expression data to be expressed in 721_B

Sample Type:
Human Hep-2 cells
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit-Alexa Fluor 568
Secondary Antibody Dilution:
1:400
Color/Signal Descriptions:
Red: IMPDH2 Blue: DAPI
Gene Name:
IMPDH2
Submitted by:
S. John Calise, Edward Chan Lab, University of Florida

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IMPDH2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
• Peptide Sequence: Synthetic peptide located within the following region: MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVD
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-IMPDH2 (ARP54365_P050) antibody is Catalog # AAP54365 (Previous Catalog # AAPP43213)
• Sample Type Confirmation:

  IMPDH2 is supported by BioGPS gene expression data to be expressed in 721_B

• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

Target Info

• Gene Symbol: IMPDH2
• Gene Full Name: IMP (inosine 5'-monophosphate) dehydrogenase 2
• Alias Symbols: IMPD2, IMPDH-II
• NCBI Gene Id: 3615
• Protein Name: Inosine-5'-monophosphate dehydrogenase 2
• Description of Target: IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. It catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.
• Uniprot ID: P12268
• Protein Accession #: NP_000875
• Nucleotide Accession #: NM_000884
• Protein Size (# AA): 514
• Molecular Weight: 56 kDa
• Protein Interactions: RSG1; APIP; HUWE1; UBC; IMPDH2; CDK2; RPA1; RPA3; RPA2; PRKAR2A; IMPDH1; EIF2S3; PARK2; MAPK10; SOX2; NPM1; PEX14; FN1; RPLP0P6; RPS6; RPL15; FOLR1; FBXO6; GRB2; UBQLN4; PIAS4; COPS5; COPS6; CUL3; YWHAZ; FBXO25; SIRT7; HDAC5; MME; SUMO4; AKT1; IKBKE; ATG5