H2AFY Antibody - N-terminal region (ARP58284_P050)

Catalog No: ARP58284_P050

Host: Rabbit
Target Name: H2AFY
Sample Type: MCF7
Antibody Dilution: 1.0ug/mlH2AFY is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human H2AFY
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 93%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: HPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARDNKKGRVTPRHILLAV
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-H2AFY (ARP58284_P050) antibody is Catalog # AAP58284 (Previous Catalog # AAPP32883)
• Sample Type Confirmation:

  H2AFY is strongly supported by BioGPS gene expression data to be expressed in MCF7

• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Reference: Bernstein,E., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (5), 1533-1538

Target Info

• Gene Symbol: H2AFY
• Gene Full Name: H2A histone family, member Y
• Alias Symbols: H2A.y, H2A/y, H2AFY, mH2A1, H2AF12M, MACROH2A1.1, macroH2A1.2
• NCBI Gene Id: 9555
• Protein Name: Core histone macro-H2A.1
• Description of Target: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H2AFY is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Uniprot ID: O75367
• Protein Accession #: NP_001035248
• Nucleotide Accession #: NM_001040158
• Protein Size (# AA): 371
• Molecular Weight: 40 kDa
• Protein Interactions: ERICH2; UBC; SUMO2; SUMO3; HIST1H3A; H3F3A; SUZ12; EED; RNF2; EZH2; BMI1; SRPK1; LMNA; ATF2; FN1; HIST1H3B; CENPA; APP; CBX5; CBX3; DIDO1; UBD; ATXN1L; NEDD8; ERBB2; SUMO1; HDGF; HDAC2; HDAC1; PARP1; tat; CUL3; SPOP; WDR77; PHF14; DEK; XRCC5; TOP1; RAN; H