FOXP3 Antibody - C-terminal region (OAAB01500)

Catalog No: OAAB01500

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Western blot analysis of FOXP3 Antibody (C-term) in 293, Jurkat cell line and mouse liver tissue lysates (35ug/lane). FOXP3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lymph tissue reacted with FOXP3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

FOXP3 Antibody (C-term) (Cat. #OAAB01500) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
  Function: Probable transcription factor. Plays a critical role in the control of immune response.
  Cellular Location: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00089}
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FOXP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human FOXP3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Monkey
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Publications:

  NOVA1 inhibition by miR-146b-5p in the remnant tissue microenvironment defines occult residual disease after gastric cancer removal. Oncotarget. 7, 2475-95 (2016). 26673617

Target Info

• Gene Symbol: FOXP3
• Alias Symbols: JM2, AIID, IPEX, PIDX, XPID, DIETER
• NCBI Gene Id: 50943
• Protein Name: Forkhead box protein P3
• Description of Target: FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in FOXP3 gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.
• Uniprot ID: Q9BZS1
• Protein Accession #: NP_001107849.1
• Molecular Weight: 47244 Da