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TAF15 antibody - N-terminal region (ARP30112_P050)

Description of Target:
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. Its gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.
Gene Symbol:
TAF15
Official Gene Full Name:
TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Alias Symbols:
Npl3; RBP56; TAF2N; TAFII68; hTAFII68
Tissue Tool:
Find tissues and cell lines supported to express TAF15.
Protein Accession# :
NP_631961
Nucleotide Accession#:
NM_139215
Swissprot Id:
Q92804-2
Host:
Rabbit
Clonality:
Polyclonal
Protein Size (# AA):
592
Molecular Weight:
61kDa
Application:
WB, IHC
Partner Proteins:
CALR, HLA-A, PDIA3, TSHR, APOB, APP, AR, B2M, CALR, CD1D, F5, F8, FBN1, FGB, HLA-C, ITGA2B, ITGA3, ITGAV, LPL, LRP1, MBL2, MTNR1A, NKX2-1, NR3C1, PDIA2, PDIA3, PLAT, PLP1, PRF1, SCARF1, SLC2A1, SLC6A4, SUMO4, TAPBP, TF, THBS1, TSHR, VWF, APOB, APP, AR, CANX, CD1D, CD40, ELF3, EPB41, F5, FGB, LRP1, MBL2,
Immunogen:
The immunogen for anti-TAF15 antibody: synthetic peptide directed towards the N terminal of human TAF15
Product Format:
Lyophilized powder
Purification:
Affinity Purified
Complete computational species homology data:
TAF15 antibody - N-terminal region (ARP30112_P050)
Predicted Homology Based on Immunogen Sequence:
Guinea pig: 100%; Human: 100%; Rabbit: 100%; Bovine: 92%; Dog: 92%; Mouse: 92%; Pig: 92%; Rat: 92%
Datasheets / Downloads:
Printable datasheet for
anti-TAF15 antibody
- ARP30112_P050
Peptide Sequence:
Synthetic peptide located within the following region: TDSSYGQNYSGYSSYGQSQSGYSQSYGGYENQKQSSYSQQPYNNQGQQQN
Blocking Peptide:
For anti-TAF15 antibody is Catalog # AAP30112 (Previous Catalog # AAPH00288)
Key Reference:
Lee, H.J., et al., (2004) FEBS Lett. 564 (1-2), 188-198
Reconstitution and Storage:
Add 50 μl of distilled water. Final anti-TAF15 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: TAF15 antibody tested with Human Daudi Cells (ARP30112_P050)

Aviva Systems Biology is the original manufacturer of this TAF15 antibody (ARP30112_P050)

Click here to view the TAF15 antibody Western Blot Protocol

Product Datasheet Link: TAF15 antibody (ARP30112_P050)

WB Suggested Anti-TAF15 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Daudi

Western Blot image:


Description of Target: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. Its gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s TAF15 antibody (ARP30112_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

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Product Protocols: TAF15 antibody tested by IHC with human kidney (ARP30112)

Aviva Systems Biology is the original manufacturer of this TAF15 antibody.

Click here to view the TAF15 antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: TAF15 antibody (ARP30112)

IHC Information:

Rabbit Anti-TAF 15 Antibody
Catalog Number: ARP30112
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of collecting tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image:

Product Protocols: TAF15 antibody tested by IHC with human skeletal muscle (ARP30112)

Aviva Systems Biology is the original manufacturer of this TAF15 antibody.

Click here to view the TAF15 antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: TAF15 antibody (ARP30112)

IHC Information:

Rabbit Anti-TAF 15 Antibody
Catalog Number: ARP30112
Paraffin Embedded Tissue: Human Skeletal Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image: