ACTL6A Antibody - N-terminal region (ARP33350_P050)

Catalog No: ARP33350_P050

WB Suggested Anti-ACTL6A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T

Lanes:
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)
Primary Antibody Dilution:
2ug/ml
Secondary Antibody:
IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:
1: 20,000
Gene Name:
ACTL6A
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTL6A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: VDFPTAIGMVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEA
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ACTL6A (ARP33350_P050) antibody is Catalog # AAP33350 (Previous Catalog # AAPP04392)

Target Info

• Gene Symbol: ACTL6A
• Gene Full Name: Actin-like 6A
• Alias Symbols: Arp4, ACTL6, BAF53A, INO80K, ARPN-BETA
• NCBI Gene Id: 86
• Protein Name: Actin-like protein 6A
• Description of Target: This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
• Uniprot ID: O96019
• Protein Accession #: NP_004292
• Nucleotide Accession #: NM_004301
• Protein Size (# AA): 429
• Molecular Weight: 47kDa
• Protein Interactions: UBC; BMI1; HECW2; FBXO6; HDAC2; CBX5; UCHL5; ARID1B; RUVBL1; TRRAP; ARID1A; SMARCE1; SMARCC2; SMARCC1; SMARCA4; SMARCA2; UBD; GRB2; INO80E; CAND1; CUL3; CDK9; CDK2; H2AFZ; TP53; UL27; SMARCD1; HDGF; SOX2; SMARCAD1; KAT5; PBRM1; Bcl7b; INO80C; ACTR8; INO80