GFI1B antibody - N-terminal region (ARP30093_P050)

Catalog#: ARP30093_P050

• Size: 50ug

Human Jurkat

Human Heart

Human kidney

• Description of Target: GFI1B acts in the late stage of erythroid differentiation as a transcriptional repressor. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis.
• Gene Symbol: GFI1B
• Official Gene Full Name: Growth factor independent 1B transcription repressor
• Protein Accession#: NP_004179
• Nucleotide Accession#: NM_004188
• Swissprot Id: Q6T888
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 330
• Molecular Weight: 38kDa
• Application: WB, IHC
• Immunogen: The immunogen for anti-GFI1B antibody: synthetic peptide directed towards the N terminal of human GFI1B
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Horse: 100%; Human: 100%; Pig: 92%; Bovine: 83%; Mouse: 83%; Rat: 75%
• Peptide Sequence: Synthetic peptide located within the following region: MPRSFLVKSKMAHTYHQPRVQEDEPLWPPALTPVPRDQAPSNSPVLSTLF
• Blocking Peptide: For anti-GFI1B antibody is Catalog # AAP30093 (Previous Catalog # AAPH00269)
• Key Reference: Huang,D.Y., et al., (2004) Nucleic Acids Res. 32 (13), 3935-3946
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-GFI1B antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: GFI1B antibody tested with Human Jurkat Cells (ARP30093_P050)

Aviva Systems Biology is the original manufacturer of this GFI1B antibody (ARP30093_P050)

Click here to view the GFI1B antibody Western Blot Protocol

Product Datasheet Link: GFI1B antibody (ARP30093_P050)

WB Suggested Anti-GFI1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat

Western Blot image:


Description of Target: GFI1B acts in the late stage of erythroid differentiation as a transcriptional repressor. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s GFI1B antibody (ARP30093_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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Product Protocols: GFI1B antibody tested by IHC with human kidney (ARP30093)

Aviva Systems Biology is the original manufacturer of this GFI1B antibody.

Click here to view the GFI1B antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: GFI1B antibody (ARP30093)

IHC Information:

Rabbit Anti-GFI1B Antibody
Catalog Number: ARP30093
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule and renal corpuscle
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image:

Product Protocols: GFI1B antibody tested by IHC with human heart (ARP30093)

Aviva Systems Biology is the original manufacturer of this GFI1B antibody.

Click here to view the GFI1B antibody Immunohistochemistry (IHC) protocol

Product Datasheet Link: GFI1B antibody (ARP30093)

IHC Information:

Rabbit Anti-GFI1B Antibody
Catalog Number: ARP30093
Paraffin Embedded Tissue: Human Heart
Cellular Data: Myocardial cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

IHC Image: