C5 Antibody (OASA01017)

Catalog No: OASA01017 (Formerly GWB-E4B80D)

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Borate buffered saline
• Clonality: Monoclonal
• Clone: 046-10.2.2 (10-04)
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Functional assay|Western blot
  Preparation: Purified IgG prepared by affinity chromatography on Protein A
  Preservative Stabilisers: 0.1% - Sodium Azide (NaN₃)
  Approx Protein Conc: IgG concentration 1.0mg/ml
  Buffer Solutions: Borate buffered saline
• Reconstitution and Storage: -20°C
• Immunogen: Purified human C5.
• Purification: Purified IgG prepared by affinity chromatography on Protein A from ascites
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1.08 mg/ml
• Specificity: OASA01017 recognises secreted complement component 5 (C5), a 190kDa serine protease present in blood serum. It is composed of an 115kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production.
  C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex.
  In haemolysis inhibition studies it was found that OASA01017 will block C5 haemolytic activity.
  Removal of sodium azide is recommended prior to use in functional assays. Aviva recommend the use of OASA11305 for this purpose.
• Reference: 1. Karp, C. et al. (2000) Identification of complement factor 5 as a susceptibility locus for experimental allergic asthma. Nature Immun. 1: 221-226.
  2. Peng, T. et al. (2005) Role of C5 in the development of airway inflammation, airway hyperresponsiveness, and ongoing airway response. J. Clin. Invest. 115: 1590-1600.
  

Target Info

• Gene Symbol: CPAMD4, FLJ17816, FLJ17822, MGC142298, C5
• Alias Symbols: C5D, C5a, C5b, ECLZB, CPAMD4
• NCBI Gene Id: 727
• Protein Name: Complement C5
• Description of Target: MOUSE ANTI HUMAN C5
  
• Uniprot ID: P01031
• Protein Accession #: NP_001726.2
• Protein Size (# AA): 1676