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Datasheets/Manuals | Printable datasheet for anti-BAG1 antibody - OAAB01686 |
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Predicted Species Reactivity | Human |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Host | Rabbit |
Application | WB, IHC, FC, IF |
Additional Information | Function: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. |
:: | Cellular Location: Isoform 1: Nucleus. Cytoplasm. Note=Isoform 1 localizes predominantly to the nucleus Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria Tissue Location: Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level) |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This BAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-285 amino acids from the C-terminal region of human BAG1. |
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:1000 IHC-P: 1:50~100 FC: 1:10~50 IF: 1:10~50 |
Other Applications Image 1 Data | Flow cytometric analysis of K562 cells using BAG1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Gene Symbol | BAG1 |
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Alias Symbols | HAP, BAG-1, RAP46 |
NCBI Gene Id | 573 |
Protein Name | BAG family molecular chaperone regulator 1 |
Uniprot ID | Q99933 |
Protein Accession # | NP_001165886.1 |
Molecular Weight | 38879 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BAG1 Antibody - C-terminal region (OAAB01686)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "BAG1 Antibody - C-terminal region (OAAB01686)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "BAG1 Antibody - C-terminal region (OAAB01686)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BAG1 Antibody - C-terminal region (OAAB01686)"?
This target may also be called "HAP, BAG-1, RAP46" in publications.
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What is the shipping cost for "BAG1 Antibody - C-terminal region (OAAB01686)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BAG1 Antibody - C-terminal region (OAAB01686)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BAG1 Antibody - C-terminal region (OAAB01686)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "38879 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BAG1 Antibody - C-terminal region (OAAB01686)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BAG1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BAG1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BAG1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BAG1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BAG1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BAG1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.