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5-Methylcytosine (Methyl-CpG) Antibody (Clone #: 33D3)(50ug)


Anti-5-methyl cytosine AMM99021 has recently been referenced in the following publications:

Zhang, W., Lee, H.-R., Koo, D.-H. & Jiang, J. Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20, 25–34 (2008). ICC/IF, Arabidopsis 18239133

Maki, W. C. et al. Nanowire-transistor based ultra-sensitive DNA methylation detection. Biosens. Bioelectron. 23, 780–7 (2008). ChIP, Human 17936611

Gertych, A., Oh, J. H., Wawrowsky, K. A., Weisenberger, D. J. & Tajbakhsh, J. 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models. BMC Pharmacol. Toxicol. 14, 11 (2013). ICC/IF, Human 23394161

Minardi, D. et al. Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens. J. Cell. Mol. Med. 13, 2115–21 (2009). IHC, Human 18752633

Jensen, T. J., Novak, P., Eblin, K. E., Gandolfi, A. J. & Futscher, B. W. Epigenetic remodeling during arsenical-induced malignant transformation. Carcinogenesis 29, 1500–8 (2008). IP, Human 18448484

Vrba, L., Junk, D. J., Novak, P. & Futscher, B. W. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics 9, 486 (2008). IP, Human 18922183

Macaulay, E. C., Weeks, R. J., Andrews, S. & Morison, I. M. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm. Genome 22, 722–35 (2011). IP, Human 21874386

Fukuda, K. et al. Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes. J. Hum. Genet. 58, 446–54 (2013). IP, Human, Chimpanzee 23739127

Ribas, R. et al. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments. Dev. Biol. 355, 372–80 (2011). ICC/IF, Mouse 21497756

Zhang, W., Wang, X., Yu, Q., Ming, R. & Jiang, J. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res. 18, 1938–43 (2008). FISH, Papaya 18593814

Walsh, T. K. et al. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol. Biol. 19 Suppl 2, 215–28 (2010). IP, Pea aphid 20482652

Kurita, R., Arai, K., Nakamoto, K., Kato, D. & Niwa, O. Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant. Anal. Chem. 84, 1799–803 (2012). , 22263690

Kurita, R. & Niwa, O. DNA methylation analysis triggered by bulge specific immuno-recognition. Anal. Chem. 84, 7533–8 (2012). , 22880797

Tajbakhsh, J. Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells. Methods Mol. Biol. 1052, 77–88 (2013). , 23592032

Description of Target:
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
Product Format:
1 mg/ml in 10mM phosphate buffer; 150 mM NaCl; pH 7.4
Mouse IgG1
Datasheets / Downloads:
Species Reactivity:
Drosophila Melanogaster; Human; Arabidopsis; Zebra fish; Dog; Mouse; C.elegans; Rat; Pig; Chicken
Reconstitution and Storage:
Ready for usage. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Key Reference:
Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008)
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