REPIN1 ELISA Kit (Mouse) (OKCD01001)

Catalog No: OKCD01001 (Formerly GWB-KBBYQ1)

Size:96WELLS

Mouse Repin1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Initiator 1 (REPIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Replication Initiator 1 (REPIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Replication Initiator 1 (REPIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Replication Initiator 1 (REPIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Replication Initiator 1 (REPIN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Replication Initiator 1 (REPIN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Repin1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Repin1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Repin1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.135 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Replication Initiator 1 (REPIN1). No significant cross-reactivity or interference between Replication Initiator 1 (REPIN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Repin1
• Gene Full Name: replication initiator 1
• Alias Symbols: A;AI425994;Ap4;E430037F08Rik;replication initiator 1;Zfp46;Zfp464;Zfp-464;Zinc finger protein 464;zinc finger protein 464 (RIP60).
• NCBI Gene Id: 58887
• Protein Name: Replication initiator 1
• Description of Target: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending (By similarity).
• Uniprot ID: Q5U4E2
• Protein Accession #: NP_001073370
• Nucleotide Accession #: NM_001079901.1
• Protein Size (# AA): 545