REN Antibody (OAGA00427)

Catalog No: OAGA00427

Size:100 ul

Renin antibody [N2C2], Internal detects REN protein by Western blot analysis.
A. 30 ug K562 whole cell lysate/extract
10 % SDS-PAGE
Renin antibody [N2C2], Internal (OAGA00427) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Renin(OAGA00427) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq]
  Positive Controls: K562
  Cellular Localization: Secreted, Membrane
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 70 and 332 of Renin (Uniprot ID#P00797)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: REN
• Gene Full Name: Renin
• Alias Symbols: RTD, HNFJ2, ADTKD4
• NCBI Gene Id: 5972
• Protein Name: Renin
• Uniprot ID: P00797
• Protein Accession #: NP_000528.1
• Molecular Weight: 45 kDa