RELN ELISA Kit (Mouse) (OKCD02852)

Catalog No: OKCD02852

Size:96WELLS

Mouse RELN ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-102%	95-103%	99-105%	85-99%
  EDTA Plasma (n=5)	97-105%	81-95%	83-101%	83-102%
  Heparin Plasma (n=5)	98-105%	80-91%	78-90%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reelin (RELN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reelin (RELN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Reelin (RELN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Reelin (RELN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-89	86
  EDTA Plasma (n=5)	96-104	101
  Heparin Plasma(n=5)	83-99	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Reelin (RELN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Reelin (RELN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RELN Microplate: 96 Wells (12 x 8 Well strips)
  • RELN Lyophilized Standard: 2
  • 100X Biotinylated RELN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 6.1 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Reelin (RL). No significant cross-reactivity or interference between Reelin (RL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Reln
• Gene Full Name: reelin
• Alias Symbols: extracellular matrix serine protease;reeler;Reeler protein;reelin;rl.
• NCBI Gene Id: 19699
• Protein Name: Reelin
• Description of Target: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
• Uniprot ID: Q60841
• Protein Accession #: NP_001297393.1
• Nucleotide Accession #: NM_001310464.1
• Protein Size (# AA): 3461