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Catalog No: OKEH04696
Size:96T
Price: $525.00
SKU
OKEH04696
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology RELB ELISA Kit (Human) (OKEH04696) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RELB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RELB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RELB captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
RELB Microplate96 Wells (12 x 8 Well Strips)
RELB Standard2
Sample Diluent1 x 20 mL
RELB Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.01 ng/mL
SpecificityNatural and recombinant Human Transcription factor RelB
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRELB
Gene Full NameRELB proto-oncogene, NF-kB subunit
Alias SymbolsIMD53;IREL;I-REL;REL-B;transcription factor RelB;v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3);v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3.
NCBI Gene Id5971
Protein NameQ01201
Description of TargetTranscription factor RelB
Uniprot IDhttps://www.uniprot.org/uniprot/Q01201
Protein Accession #NP_006500.2
Nucleotide Accession #NM_006509.3
Protein Size (# AA)579
Molecular Weight62 kDa
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