RELA ELISA Kit (Human) (OKEH04558)

Catalog No: OKEH04558

Size:96T

Human RELA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology RELA ELISA Kit (Human) (OKEH04558) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RELA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for RELA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RELA captured in well.
• ELISA Kit Range: 1.56-100ng/ml
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-RELA Microplate: 96 Wells (12 x 8 Well Strips)
  • RELA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • RELA Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.89 ng/mL
• Specificity: Natural and recombinant Human Transcription factor p65
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RELA
• Gene Full Name: RELA proto-oncogene, NF-kB subunit
• Alias Symbols: CMCU;NF-kappa-B p65delta3;NF-kappa-B transcription factor p65;NFKB3;nuclear factor NF-kappa-B p65 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;p65;transcription factor p65;v-rel avian reticuloendotheliosis viral oncogene homolog A.
• NCBI Gene Id: 5970
• Protein Name: Q04206
• Description of Target: Transcription factor p65
• Uniprot ID: https://www.uniprot.org/uniprot/Q04206
• Protein Accession #: NP_001138610.1
• Nucleotide Accession #: NM_001145138.1
• Protein Size (# AA): 551
• Molecular Weight: 60 KDa