REG4 ELISA Kit (Human) (OKEH04590)

Catalog No: OKEH04590 (Formerly GWB-MRLDTP)

Size:96T

Human REG4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology REG4 ELISA Kit (Human) (OKEH04590) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for REG4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for REG4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample REG4 captured in well.
• ELISA Kit Range: 0.469-30ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 108%
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 6.1%
  Mean Inter-Assay CV: < 7.4%
• ELISA Kit Component:
  • Anti-REG4 Microplate: 96 Wells (12 x 8 Well Strips)
  • REG4 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • REG4 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.235 ng/mL
• Specificity: Natural and recombinant Human Regenerating islet-derived protein 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: REG4
• Gene Full Name: regenerating family member 4
• Alias Symbols: gastrointestinal secretory protein;GISP;reg IV;REG-4;regenerating gene type IV;regenerating islet-derived family, member 4;regenerating islet-derived protein 4;regenerating islet-derived protein IV;REG-IV;REG-like protein;RELP.
• NCBI Gene Id: 83998
• Protein Name: Q9BYZ8
• Description of Target: Regenerating islet-derived protein 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q9BYZ8
• Protein Accession #: NP_001152824.1
• Nucleotide Accession #: NM_001159352.1
• Protein Size (# AA): 158
• Molecular Weight: 18 KDa