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Catalog No: OKEH00381
Size:96T
Price: $600.00
SKU
OKEH00381
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REG3G ELISA Kit (Human) : 96 Wells (OKEH00381)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology REG3G ELISA Kit (Human) (OKEH00381) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for REG3G has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for REG3G is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample REG3G captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
REG3G Microplate96 Wells (12 x 8 Well strips)
REG3G Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated REG3G Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.78 ng/mL
SpecificityNatural and recombinant Human Regenerating islet-derived protein 3-gamma
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolREG3G
Gene Full Nameregenerating family member 3 gamma
Alias SymbolsLPPM429;pancreatitis-associated protein 1B;pancreatitis-associated protein IB;PAP IB;PAP1B;PAP-1B;PAPIB;REG III;reg III-gamma;REG-3-gamma;regenerating gene III;regenerating islet-derived 3 gamma;regenerating islet-derived protein 3-gamma;regenerating islet-derived protein III-gamma;REG-III;UNQ429.
NCBI Gene Id130120
Protein NameQ6UW15
Description of TargetRegenerating islet-derived protein 3-gamma
Uniprot IDhttps://www.uniprot.org/uniprot/Q6UW15
Protein Accession #NP_001008388.1
Nucleotide Accession #NM_001008387.2
Protein Size (# AA)175
Molecular Weight19 kDa
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