Catalog No: OKEH03109
Size:96T
Price: $525.00
SKU
OKEH03109
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03109
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology REG3B ELISA Kit (Mouse) (OKEH03109) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Reg3b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Reg3b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Reg3b captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 117%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.4% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Reg3b Microplate96 Wells (12 x 8 Well strips)
Reg3b Lyophilized Standard2
100X Biotinylated Reg3b Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.424 ng/mL
SpecificityNatural and recombinant Mouse Regenerating islet-derived protein 3-beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolReg3b
Gene Full Nameregenerating islet-derived 3 beta
Alias SymbolsHIP;P;pancreatitis-associated protein 1;Pap;PAP1;REG;reg III-beta;REG-3-beta;regenerating islet-derived protein 3-beta;regenerating islet-derived protein III-beta;REG-III;RegIII (beta).
NCBI Gene Id18489
Protein NameP35230
Description of TargetRegenerating islet-derived protein 3-beta
Uniprot IDhttps://www.uniprot.org/uniprot/P35230
Protein Accession #NP_035166.1
Nucleotide Accession #NM_011036.1
Protein Size (# AA)175
Molecular Weight19 kDa
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