Recombinant MIF (OPSD00083)

Catalog No: OPSD00083

Size:5 ug

Product Info

• Product Format: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10mM sodium phosphate (pH 7.5)
  Endotoxin Level: 0.13 EUs/ug (Kinetic LAL with 50% confidence). Acceptance Criteria: ≤1 EUs/ug
  In addition to the acceptance criteria, this product has been tested with the following assays: (1) IL-8 production in human PBMCs(2) Agarose Microdroplet Assay with U937 cells
• Reconstitution and Storage: Shipped at room temperature. Prior to reconstitution, store at -20C. Centrifuge vial before opening. Reconstitute with sterile water at 0.1 mg/mL. Suspend the product by gently pipetting the recommended solution down the sides of the vial. Do not vortex. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution. Store at -80C and avoid repeated freeze/thaw cycles.
  Expiration Date: 12 months from date of receipt when stored at -20C to -80C as supplied, 1 month when stored at 4C after reconstituting as directed, 3 months when stored at -20C to -80C after reconstituting as directed.
• Purity: >95% (Reducing & Non-Reducing SDS-PAGE) Acceptance Criteria: ≥95%
• Biological Activity: Bioactive protein, but no acceptance criteria established.
• Protein Sequence: MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA
  
• Source: Genetically modified E.coli

Target Info

• Gene Symbol: AMH
• Alias Symbols: MIF, MIS
• NCBI Gene Id: 268
• Protein Name: Muellerian-inhibiting factor
• Description of Target: Macrophage migration inhibitory factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity. MIF promotes fibroblast migration by inducing interleukin 1 (IL-1), interleukin 8 (IL-8), and matrix metalloproteinase (MMP) expression. In interferon-gamma-activated macrophages, MIF stimulates nitric oxide (NO) production and tumor necrosis factor-alpha (TNF-alpha) secretion.
• Uniprot ID: P03971
• Molecular Weight: Monomer, 12.5 kDa (115aa)