HUMAN M-CSF Recombinant Protein (10ug)

Catalog#: OPSA11066

• Size: 10ug

• Description of Target: RECOMBINANT HUMAN M-CSF
• Gene Symbol: CSF1
• Alias Symbols: MCSF; MGC31930; CSF1
• Protein Accession#: NP_000748.3
• Swissprot Id: P09603
• Protein Size (# AA): 554
• Molecular Weight: 36.8kD (159 amino acid homodimer)
• Product Format: Purified recombinant protein - lyophilised
• Purity: >98% by SDS PAGE and HPLC analysis
• Specificity: M-CSF (Macrophage colony-stimulating factor) is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.
  
  
  Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation.
• Applications Info: ELISA: 0.2 - 0.4ng/well
  Western Blotting: 1.5 - 3.0ng/lane
  Functional Assays: 1.0 - 10.0ng/ml
  
• Additional Information: Activity: The ED₅₀, determined by the dose-dependent stimulation of the proliferation of M-NSF-60 cells, was <= 1 ng/ml corresponding to a specific activity of >= 1 x 10⁶ units/mg.
  
  Approx Protein Conc: 0.1mg/ml after reconstitution.
  Endotoxin Level: < 1EU/ug
  Label Protein Molar Ratio: Purified recombinant M-CSF expressed in E.coli
  
  Preservative: None present
• Reconstitution and Storage: Reconstitution: Reconstitute with 0.1ml distilled water
  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently mixed after reconstitution.
  
  Prior to reconstitution store at +4C.
  After reconstitution store at -20C.
  Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
  Shelf Life: 3 months from date of reconstitution.