RECK ELISA Kit (Human) : 96 Wells (OKEH01699)

Catalog No: OKEH01699

Size:96T

Human RECK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology RECK ELISA Kit (Human) (OKEH01699) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RECK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RECK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RECK captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.5% (n = 20)
• ELISA Kit Component:
  • RECK Microplate: 96 Wells (12 x 8 Well strips)
  • RECK Lyophilized Standard: 2
  • 100X Biotinylated RECK Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 29 pg/mL
• Specificity: Natural and recombinant Human Reversion-inducing cysteine-rich protein with Kazal motifs
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RECK
• Gene Full Name: reversion inducing cysteine rich protein with kazal motifs
• Alias Symbols: membrane-anchored glycoprotein (metastasis and invasion);reversion-inducing cysteine-rich protein with Kazal motifs;ST15;suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs);suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs);suppressor of tumorigenicity 15 protein.
• NCBI Gene Id: 8434
• Protein Name: O95980
• Description of Target: Reversion-inducing cysteine-rich protein with Kazal motifs
• Uniprot ID: https://www.uniprot.org/uniprot/O95980
• Protein Accession #: NP_066934.1
• Nucleotide Accession #: NM_021111.2
• Protein Size (# AA): 971
• Molecular Weight: 106 kDa