Catalog No: OKCA01036
Size:96 Wells
Price: $857.00
SKU
OKCA01036
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8882-95
1:29894-102
1:49790-105
1:88682-92
ELISA Kit PrincipleAviva Systems Biology RBP3 ELISA Kit (Bovine) (OKCA01036) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RBP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RBP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RBP3 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9088-96
EDTA Plasma (n=4)10096-103
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level OKCA01036 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01036 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
RBP3 Microplate96 Wells (12 x 8 Well strips)
RBP3 Lyophilized Standard2 x 10 ng
100X Biotinylated RBP3 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.039 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolRBP3
Gene Full NameRetinol-binding protein 3
Alias SymbolsD10S64, D10S65, D10S66, IRBP, RBPI, interphotoreceptor retinoid-binding protein|retinol-binding protein 3|retinol-binding protein 3, interstitial
NCBI Gene Id281443
Protein NameRetinol-binding protein 3
Description of TargetIRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.
Uniprot IDP12661
Protein Accession #NP_776589.1
Nucleotide Accession #NM_174164.1
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