RBM35B Antibody (OAPB01253)

Catalog No: OAPB01253

Size:100UG

Western blot analysis of RBM35B in human lung tissue lysate with RBM35B antibody at 1 μg/mL.

Immunohistochemistry of RBM35B in human lung tissue with RBM35B antibody at 5 μg/mL.

Immunofluorescence of RBM35B in human lung tissue cells with RBM35B antibody at 20 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. RBM35B Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: RBM35B antibody can be stored at 4C for three months and -20C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: RBM35B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human RBM35B. The immunogen is located within the last 50 amino acids of RBM35B.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Specificity: RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least three isoforms of RBM35B are known to exist; this antibody will detect all three.
• Application Info: RBM35B antibody can be used for detection of RBM35B by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL..
  POSITIVE CONTROL: Cat. No. OCPB00148 - Human Lung Tissue Lysate
• Reference: Warzecha CC, Sato TK, Nabet B, et al. ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing. Mol Cell. 2009; 33:591-601.;Warzecha CC, Jiang P, Amirikian K, et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J. 2010; 29:3286-300. ;Warzecha CC, Shen S, Xing Y, et al. The epithelial splicing factors ESRP1 and ESRP2 positively and negatively regulate diverse types of alternative splicing events. RNA Biol. 2009; 6:546-62.;

Target Info

• Gene Symbol: ESRP2
• Gene Full Name: epithelial splicing regulatory protein 2
• Alias Symbols: epithelial splicing regulatory protein 2;RBM35B;RNA binding motif protein 35A;RNA-binding motif protein 35B;RNA-binding protein 35B.
• NCBI Gene Id: 80004
• Protein Name: Epithelial splicing regulatory protein 2|epithelial splicing regulatory protein 2 [Homo sapiens]
• Description of Target: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
• Uniprot ID: Q9H6T0
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_079215