RBM15 Antibody (OAGA00805)

Catalog No: OAGA00805

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
OAGA00805 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBM15(OAGA00805) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM]
  Positive Controls: A431, H1299, HeLa
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 146 and 425 of RBM15 (Uniprot ID#Q96T37)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.57 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RBM15
• Gene Full Name: Putative RNA-binding protein 15
• Alias Symbols: OTT, OTT1, SPEN
• NCBI Gene Id: 64783
• Protein Name: Putative RNA-binding protein 15
• Uniprot ID: Q96T37
• Protein Accession #: NP_001188474.1
• Molecular Weight: 107 kDa