- Description of Target:
- RBBP9 may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate.
- Gene Symbol:
- Official Gene Full Name:
- Retinoblastoma binding protein 9
- NCBI Gene Id:
- Alias Symbols:
- BOG, MGC9236, RBBP10
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express RBBP9.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express RBBP9.
- Swissprot Id:
- Protein Accession #:
- Nucleotide Accession #:
- Protein Name:
- Putative hydrolase RBBP9
- Protein Size (# AA):
- Molecular Weight:
- IF, WB
- Protein Interactions:
- RB1; TERF2IP; UBC; YRDC; RBL2; RBL1;
- The immunogen is a synthetic peptide directed towards the N terminal region of human RBBP9
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Affinity Purified
- Complete computational species homology data:
- Anti-RBBP9 (ARP34703_P050)
- Predicted Homology Based on Immunogen Sequence:
- Cow: 79%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%; Zebrafish: 91%
- Species Reactivity:
- Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
- Datasheets / Downloads:
- Printable datasheet for anti-RBBP9 (ARP34703_P050) antibody
- Peptide Sequence:
- Synthetic peptide located within the following region: MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITA
- Blocking Peptide:
- For anti-RBBP9 (ARP34703_P050) antibody is Catalog # AAP34703 (Previous Catalog # AAPP23651)
- Target Reference:
- Chen,J., (2003) J. Hum. Genet. 48 (4), 164-169
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Customer Reviews for RBBP9 Antibody (ARP34703_P050) tested with corneal endothelium in Immunofluorescence
CAT# ARP34703_P050 RBBP9
Submitted By: Laboratory of Biology & Pathology Institute of the Eye, Charles University in Prague
Cryosections: - control corneal buttons were dissected, snap frozen in liquid nitrogen, embedded in Optimal Cutting Temperature Compound and stored at 70Â°C. Tissue slices 7Î¼m thick were cut as cryosections.
Method: Indirect immunofluorescence
Three cryosections on each slide were stained with a single antibody. The fourth slice was used as a negative control (primary antibody omitted). The tissue was fixed with cold acetone for 10 minutes, rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature.
Rabbit anti human antibodies and dilutions used:
f) RBBP9, most suitable concentration 1:150
Then the specimens were washed three times in PBS and incubated with the secondary antibody (fluorescein isothiocyanate-conjugated anti-rabbit IgG, 1:300, Jackson ImmunoResearch Laboratories, West Grove, USA) for 1 hour at room temperature. After rinsing in PBS the slices were mounted with Vectashield-propidium iodide (Vector Laboratories, Inc. Burlingame, USA) to counterstain nuclear DNA.
RBBP9 - corneal endothelium, epithelium as well as limbal epithelium and stromal keratocytes were positive (strong intracytoplasmic positivity â€“ â€œdotsâ€ within cell cytoplasm, weaker signal in the nucleus).
Aviva Systems Biology is the original manufacturer of this RBBP9 antibody (ARP34703_P050)
Click here to view the RBBP9 antibody Western Blot Protocol
Product Datasheet Link: RBBP9 antibody (ARP34703_P050)
WB Suggested Anti-RBBP9 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: THP-1
Western Blot image:
Description of Target: RBBP9 may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate.
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