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Size:	0.1 mg
Price:	$385.00
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Rat Anti- Mouse CD40- SPRD(0.1 mg)

Catalog#: OASB00443
Domestic: within 1- 2 weeks delivery International: 1- 2 weeks

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BALB/c spleen

CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs.^1-5 CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow, with the levels of CD40 expression increasing thereafter during B-cell maturation. Immature B cells (IgM⁺IgD^loB220^lo) express intermediate levels of CD40, whereas mature B cells (IgM⁺IgD^hiB220^hi) express high levels.⁴ CD40 has a central role in B cell growth and differentiation, and signalling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE.⁶ The agonistic 1C10 antibody closely resembles gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors.²

Host:: Rat
Specificity:: Mouse CD40, Mr 45- 50 kDa
Application:: FC
Warning:: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution:: Flow Cytometry: Fluorescein conjugate <= 2 ug/10⁶ cells
Biotin conjugate <= 2 ug/10⁶ cells
R-phycoerythrin conjugate <= 0.5 ug/10⁶ cells
Allophycocyanin conjugate <= 0.5 ug/10⁶ cells
Spectral Red conjugate <= 0.5 ug/10⁶ cells
Clone:: 1C10
Product Format:: SpectralRed (SPRD) Conjugate

Isotype:: IgG2a
Applications Info:: - Flow cytometry^7,9
- Immunoprecipitation ⁸
- In vitro induction of proliferation of mature B lymphocytes ⁷
Key Reference:: 1. Santos- Argumedo, L., J. Gordon, A.W. Heath, and M. Howard. 1994. Cell. Immunol. 156:272
2. Heath, A.W., W.W. Wu, and M.C. Howard. 1994. Eur. J. Immunol. 24:1828
3. Hasbold, J., C. Johnson- Leger, C.J. Atkins, E.A. Clark, and G.G. Klaus. 1994. Eur. J. Immunol. 24:1835
4. Grandien, A., A. Bras, and C. Martinez. 1996. Scand. J. Immunol. 43:47
5. Noelle, R.J., J.A. Ledbetter, and A. Aruffo. 1992. Immunol. Today 13:431
6. Clark, E.A., and J.A. Ledbetter. 1994. Nature 367:425
7. Segui, B., N. Andrieu- Abadie, S. Adam- Klages, O. Meilhac, D. Kreder, V. Garcia, A.P. Bruno., J. Jaffrezou, r. Salvayre, M. Kronke, and. T. Levade. 1999. J. Biol. Chem. 274(52):37251
8. Coope, H.J., P.G.P. Atkinson, B. Huhse, M. Belich, J. Janzen, M.J. Holman, G.G.B. Klaus, L.H. Johnston, and S.C. Ley. 2002. EMBO J
. 21(20):5375
9. Rolph, M.S., S.H.E. Kaufmann. 2001. J. Immunol. 166:5115.
Datasheets / Downloads:: OASB00443

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Rat Anti- Mouse CD40- SPRD(0.1 mg)

BALB/c spleen

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