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Catalog No: OKCD00527 (Formerly GWB-KBBHG9)
Size:96WELLS
Price: $675.00
SKU
OKCD00527
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Datasheets/ManualsPrintable datasheet for RARRES1 ELISA Kit (Human) (OKCD00527)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-90%95-103%79-91%84-102%
EDTA Plasma (n=5)96-105%98-105%84-96%82-96%
Heparin Plasma (n=5)81-89%91-99%78-99%88-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoic Acid Receptor Responder 1 (RARRES1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoic Acid Receptor Responder 1 (RARRES1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoic Acid Receptor Responder 1 (RARRES1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Retinoic Acid Receptor Responder 1 (RARRES1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)98-105102
EDTA plasma(n=5)91-10194
heparin plasma(n=5)87-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoic Acid Receptor Responder 1 (RARRES1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoic Acid Receptor Responder 1 (RARRES1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RARRES1 Microplate96 Wells (12 x 8 Well Strips)
RARRES1 Lyophilized Standard2 x 10 ng
100X Biotinylated RARRES1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Retinoic Acid Receptor Responder 1 (RARRES1). No significant cross-reactivity or interference between Retinoic Acid Receptor Responder 1 (RARRES1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRARRES1
Gene Full Nameretinoic acid receptor responder 1
Alias Symbolslatexin-like;LXNL;PERG-1;phorbol ester-induced gene 1 protein;RAR-responsive protein TIG1;retinoic acid receptor responder (tazarotene induced) 1;retinoic acid receptor responder protein 1;tazarotene-induced gene 1 protein;TIG1.
NCBI Gene Id5918
Protein NameRetinoic acid receptor responder protein 1
Description of TargetInhibitor of the cytoplasmic carboxypeptidase AGBL2, may regulate the alpha-tubulin tyrosination cycle.
Uniprot IDP49788
Protein Accession #NP_002879
Nucleotide Accession #NM_002888.3
Protein Size (# AA)294
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