RARA ELISA Kit (Human) : 96 Wells (OKEH02882)

Catalog No: OKEH02882

Size:96T

Human RARA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology RARA ELISA Kit (Human) (OKEH02882) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RARA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RARA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RARA captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.4% (n = 20)
  Mean Inter-assay CV%: < 9.7% (n = 20)
• ELISA Kit Component:
  • RARA Microplate: 96 Wells (12 x 8 Well strips)
  • RARA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated RARA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.13 ng/mL
• Specificity: Natural and recombinant Human Retinoic acid receptor alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RARA
• Gene Full Name: retinoic acid receptor alpha
• Alias Symbols: NR1B1;nuclear receptor subfamily 1 group B member 1;nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form;PML-DDX5-RARA fusion;PML-DDX5-RARA fusion protein;RAR;RAR-alpha;retinoic acid nuclear receptor alpha variant 1;retinoic acid nuclear receptor alpha variant 2;retinoic acid receptor alpha;retinoic acid receptor, alpha polypeptide.
• NCBI Gene Id: 5914
• Protein Name: P10276
• Description of Target: Retinoic acid receptor alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P10276
• Protein Accession #: NP_000955.1
• Nucleotide Accession #: NM_000964.3
• Protein Size (# AA): 462
• Molecular Weight: 50 kDa