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Datasheets/Manuals | Printable datasheet for Raf1 Antibody (Phospho-Tyr341) (OAAF00108) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Tyr341. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | Raf1 (Phospho-Tyr341) antibody detects endogenous levels of Raf1 only when phosphorylated at tyrosine 341. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:1000 |
Reference | David R. Hodge, J. Biol. Chem., Jun 1998; 273: 15727. Michael A. Beazely, Mol. Pharmacol., Jan 2005; 67: 250 - 259. Deanna G. Adams, J. Biol. Chem., Dec 2005; 280: 42644 - 42654. Antonino Colanzi, J. Cell Biol., Apr 2003; 161: 27. |
Gene Symbol | RAF1 |
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Gene Full Name | Raf-1 proto-oncogene, serine/threonine kinase |
Alias Symbols | CMD1NN;c-Raf;CRAF;C-Raf proto-oncogene, serine/threonine kinase;NS5;Oncogene RAF1;proto-oncogene c-RAF;raf proto-oncogene serine/threonine protein kinase;RAF proto-oncogene serine/threonine-protein kinase;Raf-1;v-raf-1 murine leukemia viral oncogene homolog 1;v-raf-1 murine leukemia viral oncogene-like protein 1. |
NCBI Gene Id | 5894 |
Protein Name | RAF proto-oncogene serine/threonine-protein kinase |
Description of Target | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. |
Uniprot ID | P04049 |
Protein Accession # | NP_002871.1 |
Molecular Weight | 73 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)"?
This target may also be called "CMD1NN;c-Raf;CRAF;C-Raf proto-oncogene, serine/threonine kinase;NS5;Oncogene RAF1;proto-oncogene c-RAF;raf proto-oncogene serine/threonine protein kinase;RAF proto-oncogene serine/threonine-protein kinase;Raf-1;v-raf-1 murine leukemia viral oncogene homolog 1;v-raf-1 murine leukemia viral oncogene-like protein 1." in publications.
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What is the shipping cost for "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "73 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Raf1 Antibody (Phospho-Tyr341) (OAAF00108)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RAF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RAF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RAF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RAF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.