RAF1 Antibody (Phospho-Thr269) (OAAF00476)

Catalog No: OAAF00476 (Formerly GWB-ASB919)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain, using C-RAF (Phospho-Thr269) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Thr269.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: C-RAF (Phospho-Thr269) antibody detects endogenous levels of C-RAF only when phosphorylated at threonine 269.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:40000
• Reference: The MGC Project Team; Genome Res. 14:2121-2127(2004).
  Olsen J.V., Cell 127:635-648(2006).
  Mott H.R., Proc. Natl. Acad. Sci. U.S.A. 93:8312-8317(1996).

Target Info

• Gene Symbol: RAF1
• Gene Full Name: Raf-1 proto-oncogene, serine/threonine kinase
• Alias Symbols: CMD1NN;c-Raf;CRAF;C-Raf proto-oncogene, serine/threonine kinase;NS5;Oncogene RAF1;proto-oncogene c-RAF;raf proto-oncogene serine/threonine protein kinase;RAF proto-oncogene serine/threonine-protein kinase;Raf-1;v-raf-1 murine leukemia viral oncogene homolog 1;v-raf-1 murine leukemia viral oncogene-like protein 1.
• NCBI Gene Id: 5894
• Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• Description of Target: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
• Uniprot ID: P04049
• Protein Accession #: NP_002871.1
• Molecular Weight: 73 kDa