Catalog No: OKCD00426 (Formerly GWB-KBBBQ1)
Size:96WELLS
Price: $675.00
SKU
OKCD00426
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Datasheets/ManualsPrintable datasheet for RACGAP1 ELISA Kit (Human) (OKCD00426)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-102%89-97%93-101%92-105%
EDTA plasma(n=5)87-101%79-101%79-104%99-105%
heparin plasma(n=5)92-105%85-98%80-92%95-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rac-GTPase Activating Protein 1 (RACGAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rac-GTPase Activating Protein 1 (RACGAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rac-GTPase Activating Protein 1 (RACGAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rac-GTPase Activating Protein 1 (RACGAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-9882
EDTA plasma(n=5)91-10596
heparin plasma(n=5)82-8985
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rac-GTPase Activating Protein 1 (RACGAP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rac-GTPase Activating Protein 1 (RACGAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RACGAP1 Microplate96 Wells (12 x 8 Well Strips)
RACGAP1 Lyophilized Standard2 x 10 ng
100X Biotinylated RACGAP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.056 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Rac-GTPase Activating Protein 1 (RACGAP1). No significant cross-reactivity or interference between Rac-GTPase Activating Protein 1 (RACGAP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRACGAP1
Gene Full NameRac GTPase activating protein 1
Alias SymbolsCYK4;HsCYK-4;ID-GAP;male germ cell RacGap;MgcRacGAP;protein CYK4 homolog;rac GTPase-activating protein 1.
NCBI Gene Id29127
Protein NameRac GTPase-activating protein 1
Description of TargetComponent of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
Uniprot IDQ9H0H5
Protein Accession #NP_001119575
Nucleotide Accession #NM_001126103.2
Protein Size (# AA)632
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