RAC1 Antibody (OAAF01424)

Catalog No: OAAF01424 (Formerly GWB-ASC806)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells, treated with EGF 200ng/ml 30', using Rac1/CDC42 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Rac1/CDC42 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Rac1/CDC42.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Rac1/CDC42 (Ab-71) antibody detects endogenous levels of total Rac1/CDC42 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 2:10000
• Reference: Didsbury J., J. Biol. Chem. 264:16378-16382(1989).
  Drivas G.T., Mol. Cell. Biol. 10:1793-1798(1990).
  Matos P., Biochem. Biophys. Res. Commun. 277:741-751(2000).

Target Info

• Gene Symbol: CDC42|RAC1|RAC2|RAC3
• Gene Full Name: cell division cycle 42|Rac family small GTPase 1|Rac family small GTPase 2|Rac family small GTPase 3
• Alias Symbols: CDC42Hs;cell division control protein 42 homolog;cell migration-inducing gene 5 protein;dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD));dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD));EN-7;G25K;G25K GTP-binding protein;growth-regulating protein;GTP binding protein, 25kDa;Gx;HSPC022;IMD73A;IMD73B;IMD73C;MIG5;MRD48;p21-Rac1;p21-Rac2;p21-Rac3;Rac-1;ras-like protein TC25;ras-related C3 botulinum toxin substrate 1;ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1);ras-related C3 botulinum toxin substrate 2;ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2);ras-related C3 botulinum toxin substrate 3;ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3);Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2);rho family, small GTP binding protein Rac3;small G protein;small GTP binding protein CDC42;TC-25;TKS.
• NCBI Gene Id: 5879|5880|5881|998
• Protein Name: Cell division control protein 42 homolog|Ras-related C3 botulinum toxin substrate 1|Ras-related C3 botulinum toxin substrate 2|Ras-related C3 botulinum toxin substrate 3
• Description of Target: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.|Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.|Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.|Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
• Uniprot ID: P15153|P60763|P60953|P63000
• Protein Accession #: NP_008839.2
• Molecular Weight: 21 kDa