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Catalog No: OKEH02358
Size:96T
Price: $625.00
SKU
OKEH02358
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RAB8A ELISA Kit (Human) : 96 Wells (OKEH02358)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology RAB8A ELISA Kit (Human) (OKEH02358) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB8A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB8A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAB8A captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
RAB8A Microplate96 Wells (12 x 8 Well strips)
RAB8A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated NANOG Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Ras-related protein Rab-8A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRAB8A
Gene Full NameRAB8A, member RAS oncogene family
Alias SymbolsMEL;mel transforming oncogene (derived from cell line NK14);mel transforming oncogene (derived from cell line NK14)- RAB8 homolog;mel transforming oncogene (RAB8 homolog);oncogene c-mel;RAB8;ras-associated protein RAB8;ras-related protein Rab-8A.
NCBI Gene Id4218
Protein NameP61006
Description of TargetRas-related protein Rab-8A
Uniprot IDhttps://www.uniprot.org/uniprot/P61006
Protein Accession #NP_005361.2
Nucleotide Accession #NM_005370.4
Protein Size (# AA)207
Molecular Weight24 kDa
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