RAB5A Antibody - middle region (ARP56563_P050)

Catalog No: ARP56563_P050

WB Suggested Anti-RAB5A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Muscle

Host: Mouse
Target Name: RAB5A
Sample Tissue: Mouse Kidney
Antibody Dilution: 1ug/ml

Sample type: zebra gut epithelial cells
Green: primary
Red: actin
Blue: DAPI
Primary dilution: 1:5000
Secondary Dilution: 1:300
Image Submitted by: Michel Bagnat
Duke University Medical Center See Customer Feedback tab for detailed information.

Sample type: zebra gut epithelial cells
Green: primary
Red: actin
Blue: DAPI
Primary dilution: 1:5000
Secondary Dilution: 1:300
Image Submitted by: Michel Bagnat
Duke University Medical Center See Customer Feedback tab for detailed information.

Product Info

• Tested Species Reactivity: Human, Mouse, Zebrafish
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAB5A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 79%
• Peptide Sequence: Synthetic peptide located within the following region: KTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCS
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-RAB5A (ARP56563_P050) antibody is Catalog # AAP56563 (Previous Catalog # AAPP39220)
• Reference: Coyne,C.B., (2007) Cell Host Microbe 2 (3), 181-192
• Publications:

  Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells. J Extracell Vesicles. 6, 1294368 (2017). 28326171

Target Info

• Gene Symbol: RAB5A
• Gene Full Name: RAB5A, member RAS oncogene family
• Alias Symbols: RAB5
• NCBI Gene Id: 5868
• Protein Name: Ras-related protein Rab-5A
• Description of Target: RAB5A is required for the fusion of plasma membranes and early endosomes.
• Uniprot ID: P20339
• Protein Accession #: NP_004153
• Nucleotide Accession #: NM_004162
• Protein Size (# AA): 215
• Molecular Weight: 24kDa
• Protein Interactions: UBC; TNF; PTGIR; BAG3; NME2; KCNN3; SUN2; RABAC1; APPL1; ATP13A2; IGF2BP2; RAB7A; MOGS; MOV10; RHOT2; SNCA; RUFY1; RBSN; RABGEF1; ANKFY1; RABEP1; RAB11A; EEA1; TYRP1; TF; PIK3CB; PIK3C3; GRIK4; TBC1D17; TBC1D15; RIN1; KCNA5; SGSM3; RABEP2; SH3KBP1; CFTR;