RAB1A ELISA Kit (Mouse) (OKCA01761)

Catalog No: OKCA01761

Size:96 Wells

Mouse RAB1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology Rab1a ELISA Kit (Mouse) (OKCA01761) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rab1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rab1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Rab1a captured in the well.
• ELISA Kit Range: 23.5 - 1,500 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	108	103-112
  EDTA Plasma (n=4)	95	90-99

• ELISA Kit Component:
  • Rab1a Microplate: 96 Wells (12 x 8 Well strips)
  • Rab1a Lyophilized Standard: 2 x 1.5 ng
  • 100X Biotinylated Rab1a Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 5.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: RAB1A
• Gene Full Name: RAB1A, member RAS oncogene family
• Alias Symbols: Ra, Gtb, Rab, Ypt, Gtbp, Rab1, Ypt1, Rab-1, mKIAA3012
• NCBI Gene Id: 19324
• Protein Name: Ras-related protein Rab-1A
• Description of Target: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
• Uniprot ID: P62821
• Protein Accession #: NP_033022.1
• Nucleotide Accession #: NM_008996.3