Catalog No: OKCD00582 (Formerly GWB-KBBBI7)
Size:96WELLS
Price: $675.00
SKU
OKCD00582
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Datasheets/ManualsPrintable datasheet for RAB1A ELISA Kit (Human) (OKCD00582)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB1A, Member RAS Oncogene Family (RAB1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAB1A, Member RAS Oncogene Family (RAB1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB1A, Member RAS Oncogene Family (RAB1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAB1A, Member RAS Oncogene Family (RAB1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB1A, Member RAS Oncogene Family (RAB1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB1A, Member RAS Oncogene Family (RAB1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RAB1A Microplate96 Wells (12 x 8 Well Strips)
RAB1A Lyophilized Standard2 x 10 ng
100X Biotinylated RAB1A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.067 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of RAB1A, Member RAS Oncogene Family (RAB1A). No significant cross-reactivity or interference between RAB1A, Member RAS Oncogene Family (RAB1A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRAB1A
Gene Full NameRAB1A, member RAS oncogene family
Alias SymbolsGTP binding protein Rab1a;Rab GTPase YPT1 homolog;RAB1;RAB1, member RAS oncogene family;ras-related protein Rab-1A;YPT1;YPT1-related protein.
NCBI Gene Id5861
Protein NameRas-related protein Rab-1A
Description of TargetThe small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
Uniprot IDP62820
Protein Accession #NP_004152
Nucleotide Accession #NM_004161.4
Protein Size (# AA)205
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