RAB11A ELISA Kit (Rat) : 96 Wells (OKEH01233)

Catalog No: OKEH01233

Size:96T

Rat RAB11A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology RAB11A ELISA Kit (Rat) (OKEH01233) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat RAB11A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RAB11A and incubated. The RAB11A found in the sample or standards competes with the biotinylated RAB11A for limited binding sites on the immobilized anti-rat RAB11A antibody. Excess unbound biotinylated RAB11A and sample or standard RAB11A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RAB11A captured in the well and inversely proportional to the amount of RAB11A which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.4% (n = 20)
  Mean Inter-assay CV%: < 9.4% (n = 20)
• ELISA Kit Component:
  • Anti-RAB11A Microplate: 96 Wells (12 x 8 Well strips)
  • RAB11A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X RAB11A-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.81 ng/mL
• Specificity: Natural and recombinant Rat Ras-related protein Rab-11A
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Rab11a
• Gene Full Name: RAB11a, member RAS oncogene family
• Alias Symbols: 24KG;rab-11;ras-related protein Rab-11A.
• NCBI Gene Id: 81830
• Protein Name: P62494
• Description of Target: Ras-related protein Rab-11A
• Uniprot ID: https://www.uniprot.org/uniprot/P62494
• Protein Accession #: NP_112414.1
• Nucleotide Accession #: NM_031152.2
• Protein Size (# AA): 216
• Molecular Weight: 24 kDa