Catalog No: OKEH04635
Size:96T
Price: $525.00
SKU
OKEH04635
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04635
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology PZP ELISA Kit (Human) (OKEH04635) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PZP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PZP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PZP captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-PZP Microplate96 Wells (12 x 8 Well Strips)
PZP Lyophilized Standard2
Sample Diluent1 x 20 mL
PZP Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity43 pg/mL
SpecificityNatural and recombinant Human Pregnancy zone protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPZP
Gene Full NamePZP, alpha-2-macroglobulin like
Alias SymbolsC3 and PZP-like alpha-2-macroglobulin domain-containing protein 6.
NCBI Gene Id5858
Protein NameP20742
Description of TargetPregnancy zone protein
Uniprot IDhttps://www.uniprot.org/uniprot/P20742
Protein Accession #NP_002855.2
Nucleotide Accession #NM_002864.2
Protein Size (# AA)1482
Molecular Weight164 KDa
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