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Catalog No: OKEH04431
Size:96T
Price: $525.00
SKU
OKEH04431
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04431
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Pyy ELISA Kit (Mouse) (OKEH04431) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pyy has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Pyy is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pyy captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.4% (n = 20)
Mean Inter-assay CV%: <= 8.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Pyy Microplate96 Wells (12 x 8 Well Strips)
Pyy Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Pyy Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity8.12 pg/mL
SpecificityNatural and recombinant Mouse Peptide YY
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolPyy
Gene Full Namepeptide YY
Alias SymbolsPeptide tyrosine tyrosine;peptide YY.
NCBI Gene Id217212
Protein NameQ9EPS2
Description of TargetPeptide YY
Uniprot IDhttps://www.uniprot.org/uniprot/Q9EPS2
Protein Accession #NP_663410.1
Nucleotide Accession #NM_145435.1
Protein Size (# AA)98
Molecular Weight11 KDa
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