Pyk2 Antibody (Phospho-Tyr402) (OAEC00201)

Catalog No: OAEC00201

Size:50ug

Western blot analysis of extract from Jurkat cells, untreated or treated with PMA (1ng/ml, 5min), using Pyk2 (Ab-402) antibody (OAEC00550, Lane 1 and 2) and Pyk2 (phospho- Tyr402) antibody (OAEC00201, Lane 3 and 4).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 (phospho-Tyr402) antibody (OAEC00201).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: IF, IHC, WB
• Reconstitution and Storage: Store at -20C/1 year
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Pyk2 around the phosphorylation site of tyrosine 402 (D-I-Y^P-A-E).
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation
• Concentration: 1 mg/ml
• Specificity: Pyk2 (phospho-Tyr402) antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB:1:500-1:1000, IHC: 1:50-1:100
• Reference: Gluck SL, et al. (2004) J Clin Invest; 114(12): 1696-1699 Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9784-9789 Tian D, et al. (2002) Mol Cell Biol; 22(8): 2650-2662 Lu Z, et al. (2001) Mol Cell Biol; 21(12): 4016-4031 Krishnan HH, et al. (2006) J Virol; 80(3): 1167-1180

Target Info

• Gene Symbol: PTK2B
• Gene Full Name: Protein-tyrosine kinase 2-beta
• Alias Symbols: PKB, PTK, CAKB, FAK2, PYK2, CADTK, FADK2, RAFTK
• NCBI Gene Id: 2185
• Protein Name: Protein-tyrosine kinase 2-beta
• Uniprot ID: Q14289
• Protein Accession #: NP_004094.3
• Molecular Weight: 116 kDa