Catalog No: OKEH04689
Size:96T
Price: $525.00
SKU
OKEH04689
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology PVRL1 ELISA Kit (Human) (OKEH04689) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PVRL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PVRL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PVRL1 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-PVRL1 Microplate96 Wells (12 x 8 Well Strips)
PVRL1 Lyophilized Standard2
Sample Diluent1 x 20 mL
PVRL1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Poliovirus receptor-related protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNECTIN1
Gene Full Namenectin cell adhesion molecule 1
Alias SymbolsCD111;CLPED1;ectodermal dysplasia 4 (Margarita Island type);ED4;herpes simplex virus type 1 sensitivity;herpes virus entry mediator C;herpesvirus Ig-like receptor;HIgR;HV1S;HVEC;nectin 1;Nectin cell adhesion molecule 1;nectin-1;OFC7;poliovirus receptor-like 1;poliovirus receptor-related 1 (herpesvirus entry mediator C);poliovirus receptor-related protein 1;PRR;PRR1;PVRL1;PVRR;PVRR1;SK-12.
NCBI Gene Id5818
Protein NameQ15223
Description of TargetNectin-1
Uniprot IDhttps://www.uniprot.org/uniprot/Q15223
Protein Accession #NP_002846.3
Nucleotide Accession #NM_002855.4
Protein Size (# AA)517
Molecular Weight57 KDa
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