Catalog No: OKEH03738
Size:96T
Price: $625.00
SKU
OKEH03738
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PTRF ELISA Kit (Rat) (OKEH03738) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptrf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ptrf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ptrf captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Component
ComponentAmount
Ptrf Microplate96 Wells (12 x 8 Well strips)
Ptrf Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ptrf Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.08 ng/mL
SpecificityNatural and recombinant Rat Polymerase I and transcript release factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPTRF
Gene Full Namepolymerase I and transcript release factor
Alias SymbolsCavin-1;cav-p60;Polymerase I and transcript release factor.
NCBI Gene Id287710
Protein NameP85125
Description of TargetCaveolae-associated protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/P85125
Protein Accession #NP_001099311.1
Nucleotide Accession #NM_001105841.1
Protein Size (# AA)392
Molecular Weight44 kDa
Write Your Own Review
You're reviewing:PTRF ELISA Kit (Rat) (OKEH03738)
Your Rating
We found other products you might like!