PTPRZ1 ELISA Kit (Human) (OKCD00396)

Catalog No: OKCD00396 (Formerly GWB-KBBJB7)

Size:96WELLS

Human PTPRZ1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PTPRZ1 Microplate: 96 Wells (12 x 8 Well Strips)
  • PTPRZ1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated PTPRZ1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase Receptor Type Z (PTPRZ) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PTPRZ1
• Gene Full Name: protein tyrosine phosphatase receptor type Z1
• Alias Symbols: HPTPZ;HPTPzeta;phosphacan;protein tyrosine phosphatase, receptor-type, Z polypeptide 1;protein tyrosine phosphatase, receptor-type, zeta polypeptide 1;Protein-tyrosine phosphatase receptor type Z polypeptide 1;protein-tyrosine phosphatase receptor type Z polypeptide 2;PTP18;PTPRZ;PTPZ;PTP-ZETA;receptor-type tyrosine phosphatase beta/zeta;receptor-type tyrosine-protein phosphatase zeta;RPTPB;RPTPbeta;R-PTP-zeta-2.
• NCBI Gene Id: 5803
• Protein Name: Receptor-type tyrosine-protein phosphatase zeta
• Description of Target: Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades (By similarity).
• Uniprot ID: P23471
• Protein Accession #: NP_001193767
• Nucleotide Accession #: NM_001206838.1
• Protein Size (# AA): 2315