PTP4A3 ELISA Kit (Human) (OKCD00913)

Catalog No: OKCD00913 (Formerly GWB-KBBHR5)

Size:96WELLS

Human PTP4A3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PTP4A3 Microplate: 96 Wells (12 x 8 Well Strips)
  • PTP4A3 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PTP4A3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.118 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PTP4A3
• Gene Full Name: protein tyrosine phosphatase 4A3
• Alias Symbols: phosphatase of regenerating liver 3;potentially prenylated protein tyrosine phosphatase;PRL3;PRL-3;PRL-R;protein tyrosine phosphatase type IVA 3;protein tyrosine phosphatase type IVA, member 3;Protein-tyrosine phosphatase 4a3;protein-tyrosine phosphatase of regenerating liver 3.
• NCBI Gene Id: 11156
• Protein Name: Protein tyrosine phosphatase type IVA 3
• Description of Target: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.
• Uniprot ID: O75365
• Protein Accession #: NP_009010
• Nucleotide Accession #: NM_007079.3
• Protein Size (# AA): 173