PTMA ELISA Kit (Human) (OKEH01154)

Catalog No: OKEH01154

Size:96T

Human PTMA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PTMA ELISA Kit (Human) (OKEH01154) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PTMA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PTMA and incubated. The PTMA found in the sample or standards competes with the biotinylated PTMA for limited binding sites on the immobilized anti-human PTMA antibody. Excess unbound biotinylated PTMA and sample or standard PTMA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PTMA captured in the well and inversely proportional to the amount of PTMA which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.1% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-PTMA Microplate: 96 Wells (12 x 8 Well strips)
  • PTMA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X PTMA-Biotin Complex: 1 x 60 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.039 ng/mL
• Specificity: Natural and recombinant Human Prothymosin alpha
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: PTMA
• Gene Full Name: prothymosin alpha
• Alias Symbols: gene sequence 28;prothymosin alpha;prothymosin alpha protein;prothymosin-I+/-;TMSA.
• NCBI Gene Id: 5757
• Protein Name: P06454
• Description of Target: Prothymosin alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P06454
• Protein Accession #: NP_001092755.1
• Nucleotide Accession #: NM_001099285.1
• Protein Size (# AA): 111
• Molecular Weight: 12 kDa