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Catalog No: OAAF00055 (Formerly GWB-ASB071)
Size:100 ug
Price: $344.00
SKU
OAAF00055
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for PTK2B Antibody (Phospho-Tyr580) (OAAF00055)
Product Info
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLiquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human PYK2 around the phosphorylation site of Tyr580.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
SpecificityPYK2 (phospho-Tyr580) antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 580.
DilutionThe optimal working dilution should be determined by the end user.
Application InfoWB 1:500~1000
IHC 1:50~100
ELISA 1:40000
ReferenceYoshiko Banno, J. Biol. Chem., Apr 2005; 280: 16319 - 16324.
Robin R. Hodges, Invest. Ophthalmol. Vis. Sci., Aug 2006; 47: 3352 - 3359.
Sarah J. McLeod, J. Biol. Chem., Mar 2004; 279: 12009 - 12019.
Chong-Shan Shi, J. Biol. Chem., Aug 2000; 27
Gene SymbolPTK2B
Gene Full Nameprotein tyrosine kinase 2 beta
Alias SymbolsCADTK;CAKB;CAK-beta;calcium-dependent tyrosine kinase;calcium-regulated non-receptor proline-rich tyrosine kinase;cell adhesion kinase beta;FADK 2;FADK2;FAK2;focal adhesion kinase 2;PKB;proline-rich tyrosine kinase 2;protein kinase B;protein-tyrosine kinase 2-beta;PTK;PTK2B protein tyrosine kinase 2 beta;PYK2;RAFTK;related adhesion focal tyrosine kinase.
NCBI Gene Id2185
Protein NameProtein-tyrosine kinase 2-beta
Description of TargetNon-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
Uniprot IDQ14289
Protein Accession #NP_004094.3
Molecular Weight115 kDa
  1. What is the species homology for "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)" provided in?

    This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)"?

    This target may also be called "CADTK;CAKB;CAK-beta;calcium-dependent tyrosine kinase;calcium-regulated non-receptor proline-rich tyrosine kinase;cell adhesion kinase beta;FADK 2;FADK2;FAK2;focal adhesion kinase 2;PKB;proline-rich tyrosine kinase 2;protein kinase B;protein-tyrosine kinase 2-beta;PTK;PTK2B protein tyrosine kinase 2 beta;PYK2;RAFTK;related adhesion focal tyrosine kinase." in publications.

  5. What is the shipping cost for "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "115 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "PTK2B Antibody (Phospho-Tyr580) (OAAF00055)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "PTK2B"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "PTK2B"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "PTK2B"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "PTK2B"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "PTK2B"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "PTK2B"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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